Yes, strollers are permitted. Wagons, however, are not permitted at Anime Expo.
Please be aware that the only designated stroller parking area is located in the back of the Main Events Hall. For panels, strollers cannot block aisles or exits, please put the stroller against the wall or in the back of the panel room and have your child sit on your lap or in an adjacent seat.
Want to learn more? Check out our Quick Guide to Attending Anime Expo with Kids for more information.